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Further implicating 4-HNE adduction to tubulin as a possible contributor to age-related pathologies was the fact that 35 M H 2 O 2 exposure resulted in an increase in 4-HNE adduction to -, -, and -tubulin as detected via PLA fluorescent intensity analysis

In this model we exposed BALB/c mice to Alternaria alternata and treated them with melatonin both at 17:00, since this is a time point that has been found to result in the most severe induction of features of allergic airway pathology ( in vivo

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Mol Cancer Ther 5:2512-2521, 2006

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