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Besides, to further confirm peroxynitrite-mediated activation of the NF-B pathway, we also measured the mRNA expression levels of known NF-B p65 target genes Bax , P53 , Bcl2 , and Bcl-xL

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For some other types of affinity purifications, like Nickel Beads and His-tags, regeneration means that we actually strip the nickel ion off of the beads and regenerate them by adding new nickel onto the beads

May have mitotic figures and nuclear atypia