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These inconsistent results, appeared in the literature, concerning the responsiveness to in ovo injection of L-carnitine might have been resulted from many factors such as differences in strain and age of breeder hens, injection technique, site of in ovo injection, timing of injection (incubational age), dose and purity of L-carnitine used, diluent type in which L-carnitine is dissolved, incubator conditions, diet composition and metabolic fate of L-carnitine

It works by blocking the action of adrenaline on the heart and blood vessels, thereby reducing heart rate, blood pressure, and the workload on the heart

The mixture was extracted with toluene, and the absorbance of the fraction with toluene aspired from the liquid phase was read at 520 nm

, Besbes S., Hentati B., Blecker C., Deroanne C., and Attia H

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