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GSH depletion causes abnormalities in GPX4, leading to excessive accumulation of lipid peroxides

Researchers comparing analogs should understand where each fits in the experimental toolkit

A Multiskan FC Microplate Reader (BELL Analytical Instruments Co., Ltd.) was used to measure absorbance at 415 nm, and an H 2 O 2 concentration gradient standard curve was used to determine the H 2 O 2 content of the sample (24)

Generation and quantification of LMW persulfides and polysulfides using GSH GSH (10 M) in 100 L 50 mM HEPES buffer (pH 7.4) was exposed to increasing concentration (010 M) of DTNB for 20 min and then reacted with Na 2 S or Na 2 S 2 (0 - 30 M) for 5 min

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