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For best results, you might allow the vial to sit at room temperature for a few minutes before reconstitution to avoid a thermal shock to the lyophilized powder, but the water itself will work just as effectively.

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For multi-dose protocols beyond 28 days, reconstitute a fresh vial and discard the previous solution

Sarvari, A

NR restores the age-associated decline in the metabolic cycle and circadian behavioral, including the BMAL1 activity, oscillation mitochondrial respiration, rhythmic transcription and late evening activity to youthful levels