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The following sgRNAs for GCLC CRISPRCas9 KO were used: sgNTC forward: 5-CACCGGAGGCTAAGCGTCGCAA-3

Indeed, this persistent immune activation may eventually lead to immune cell exhaustion and a profound depression of antitumor surveillance [165]

208 In addition, in some recent omics studies, 209 , 210 , 211 , 212 machine learning methods have been used to screen diagnostic markers from omics data