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Cells were then centrifuged, and the resulting cell pellets were resuspended in DMEM (without phenol red and serum) containing DAF-FM-DA (10 M, for NO detection), DCFH-DA (10 M, for ROS detection) or BODIPY-581/591-C11 (10 M, for lipid-ROS detection)

Older adults may be at higher risk because age-related muscle loss can already be present before treatment begins