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Subsequently, the cells were seeded in a 6-well plate at a density of 3 10 6 cells per well

In the B16F10 melanoma model, directing anti-CTLA4 and anti-PD-1 treatment to the TDLN reduces the risk of immune-related adverse events, reduces distant metastases and recurrence, and enhances the anti-cancer T cell repertoire (233)

Many growth factors such as transforming growth factor (TGF)-,2 platelet-derived growth factor (PDGF),3 epidermal growth factor (EGF),4 and fibroblast growth factor (FGF),5 as well as interleukin (IL)-16 and tumor necrosis factor (TNF),7 have been evaluated in a wide spectrum of in vivo and in vitro experiments to observe their effects on different elements included in the healing process