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For nuclei extraction, the cells were incubated with 0.1% (v/v) digitonin (Promega, USA) for 3 min at 4 C and centrifuged for 10 min at 500 x g at 4 C with 1 mL ATAC-RSB, containing 0.1% (v/v) Tween-20

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13), indicating that the polyP scaffold was maintained for the integrity of droplet under -ray irradiation

These supplements not only provide essential nutrients but also help in regulating blood glucose levels, demonstrating potential applications in functional foods and supplements for diabetes management [162]

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