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After a series of carefully controlled washing steps, the bound insulin is detected by adding a substrate that produces a detectable signal, typically a colored product, in proportion to the amount of insulin present

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PMID: 38245643

In addition, NRXs directly interact with GABA A Rs, thus modulating GABAergic transmission in a NL independent fashion (Zhang et al., 2010)

However, whether these molecular pathways contribute to the reduction of oxidative stress by NAC in LE-induced PCOS mouse ovaries in our study, or if other molecular pathways are involved, remains to be investigated