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In mitochondria, THF is imported via a mitochondrial folate transporter and converted into 5,10-methylene-THF by serine hydroxymethyltransferase 2 (SHMT2), which oxidizes serine to glycine while transferring a one-carbon unit to THF [15]

These acquisition methods can be combined with different quantitative approaches, either label-free quantification (LFQ) or label-based techniques 140,141

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Conversely, overexpression of Slc7a11, which increases GSH levels, protects against cardiac ferroptosis in ferritin H-deficient animals, suggesting a critical role for ferritin H in preventing HF (125,157)