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To better understand how changes in intracellular GSH metabolism might contribute to the expansion, survival, and function of CD8 + T cells upon chronic antigenic stimulation, we used an in vitro system (26) whereby activated transgenic or wild-type (WT) T cells were expanded in the presence of persistent antigenic stimulation with either specific antigen (OVA-I/gp100) or anti-CD3mediated stimulation and anti-CD28 costimulation for 7 days

For the shRNA KD ChIP-seq data sequenced by Novogene, the raw sequencing reads were trimmed by Cutadapt and then mapped by Bowtie 2 with the --very-sensitive parameter

T-REX coupled with co-immunoprecipitation assays revealed that NCBP1-HNEylation caused no perturbation of the association between NCBP1 and ALYREF, PHAX or PRPF4 (Supplementary Fig

doi: 10.1038/s41598-020-67383-x 77

Microbial diversity is fundamental to maintaining gut ecosystem homeostasis and overall host health, with its abundance and stability directly shaping the adaptability of the intestinal microecosystem and host physiological function