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Our results indicated that ROS levels were significantly greater in the cystine plus gentamicin group than in the gentamicin alone group (Figure 6A)

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During our previous characterization of molecular pathways leading to wheat resistance to Hessian fly using microarrays, we have found that the transcript levels of a large number of genes encoding various enzymes involved in glutathione metabolism are strongly affected in resistant plants after Hessian fly attack 21,27 , indicating that glutathione may play an important role in wheat defense against Hessian fly attack

According to the literature, H 2 O 2 can activate the TRPM2 ion channels in macrophages, promoting calcium influx and mediating immune responses 42,43,44