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For one-cycle binding measurements of AETX-R114 to biotinylated ligands HLA-A*3 (Kactus, MHC-HM44RB) or HLA-A*11 (Kactus, MHC-HM41RB) loaded with soto-p 7 , p 7 WT or G12W mutated peptide p 7 W12 , biotinylated ligands were captured on a Series S high-affinity streptavidin (SA) sensor chip, and AETX-R114, diluted in 1xHBS-EP+ to a final concentration of 80 nM, was injected into all 6 flow cells at 25 C at a flow rate of 30 L/min

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We used propensity score analysis to balance baseline characteristics for each study outcome respectively