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Particularly, how iron overload influences the neuroinflammatory microenvironment through epigenetic modifications (e.g., DNA/RNA methylation) requires validation through the establishment of a multi-omics integrative analysis framework

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5B), the time required for eliminating 25, 50, 75, and 95% H 2 O 2 at initial concentrations of 5200 M ranged as 2.02.1, 4.95.2, 1011, and 2125 min, respectively

On the following day, scopolamine methyl bromide (1 mg/kg body weight, Sigma-Aldrich) was intraperitoneally injected to minimize peripheral cholinergic effects, then 30 min later, pilocarpine hydrochloride (10 mg/kg body weight, Sigma-Aldrich) was administered every 30 min until the animals reached Racine stage 4 or 5 seizures