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Meanwhile, buffer containing negatively charged amino acids behaved similarly sodium acetate buffer, whereas those with positively charged amino acids (e.g., arginine and histidine) maintained a more acidic pH throughout the elution process

For this purpose, the targeted mutations of nucleotide sequences are introduced in the region of upstream open reading frames (uORFs) of the genes of interest

PMID 22440736

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It is usually reversible with high doses of pyridoxine (100 to 200 mg/day)