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Other metabolic reactions only detected in HepaRG incubations were hydroxylamine formation (MT1.4) of 4MeS-NE-BP and N,N -bis-dealkylation (MT2.5) of 4MeS-P-BP

The antioxidant activity of GSH intermediated via thiol (-SH) of its cysteine residue upon its interaction with ROS or electrophiles and the GSH oxidized to GSSG (oxidized Glutathione), the GSSG will reduce to GSH by Glutathione reductase (GR), the activity of GR is therefore very important to keep the antioxidant power of mammalian cells, while GSH/GSSG ratio is an indicator for redox balance in the cell

Proc Natl Acad Sci U S A (2009) 106(29):119138.10.1073/pnas.0812138106 39 BakerM

The change in the DNA causes the enzyme to be more thermolabile, which means that the enzyme loses its activity when exposed to moderate heat

Glutathione and glutaredoxinkey players in cellular redox homeostasis and signaling