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For analysis of disulfide bond formation by MALDI, the samples were desalted and concentrated using C18 Ziptip pipette tips (Millipore)

The glutathione reductase activity was measured by a reagent kit purchased from Nanjing Jiancheng Biotechnology Institute

doi: 10.1136/bmj.38933.585764.AE 110 PerrinRRisteLHannMWaltherAMukherjeeAHealdA

In one aspect of the invention, one or more NNMT inhibitors (such as a cation of Formula I or IA, or otherwise disclosed herein) is contacted with a cell expressing NNMT concurrently with one or more chemical entities (e.g., nicotinamide riboside, nicotinamide mononucleotide) that increase intracellular NAD+ levels to provide muscular therapy

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