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The organoids were incubated with dispase at 37 C for 30 min, after which they were washed twice with cold DMEM/F12 and collected by centrifugation (5 min, 4 C, 300 g )

Tumor necrosis factor- elevates neurite outgrowth through an NF-B-dependent pathway in cultured adult sensory neurons: Diminished expression in diabetes may contribute to sensory neuropathy