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doi: 10.1038/nmeth.1923 19 LiHHandsakerBWysokerAFennellTRuanJHomerNet al

It is believed that this increased bleeding risk is due to a alpha-tocopherol metabolite that has anti-vitamin K activity

On the subsequent day, the wells were blocked (200 L/well) with PBS/M (pH 7.4) for 1 h at room temperature and washed (250 L/well) four times with PBS/T (pH 7.4)