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Notably, Akt phosphorylation in Insr P1195L/ +/HFD mice was barely induced by insulin, indicating that insulin signaling is markedly impaired by combination of genetic mutation ( Insr P1195L ) in the insulin receptor and environmental condition (HFD feeding) in both WAT and liver

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Final DMSO concentration in cell culture should not exceed 0.1% to avoid cytotoxicity