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On the other hand, we could not detect any downregulation of microglial homeostatic genes ( p2ry12 and cx3cr1 ), possibly because it was difficult to detect differences in qRT-PCR using bulk samples

We then tested whether GR is involved in regulating DEX-induced CD82 expression by using qPCR and immunoblots

RNA interference siRNAs targeting ATXN3 were purchased from Microsynth: siATXN3 #1 UGGCAGAAGGAGGAGUUACTT (Wang et al, 2012), siATXN3 #2 CAGGGCUAUUCAGCUAAGUAUTT (Sacco et al, 2014), siATXN3 #3 GCACUAAGUCGCCAAGAAATT (Ashkenazi et al, 2017), and siATXN3 #4 GCAGGGCUAUUCAGCUAAGTT (Ashkenazi et al, 2017)

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We observed no significant changes in the expression of p53 or p21 in keloid lesions or fibroblasts compared to those in normal skin