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Two-dimensional gel electrophoresis The extracted histone proteins were dissolved in 150 l rehydration buffer (8 M urea, 2% CHAPS, 0.5% IPG buffer and 0.002% bromophenol blue), and then loaded onto isoelectric focusing (IEF) strips using the following programme: 20 V for 10 h (rehydration)

For this, rats were randomly divided into the following three groups: (1) control group

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