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Generation of I 1 r Pf GS, I 2 r Pf GS and I 1 I 2 r Pf GS recombinant plasmids To generate I 1 r Pf GS and I 2 r Pf GS recombinant plasmids, the two fragments on either side of the respective peptide inserts were PCR amplified individually and cloned sequentially into pRSETA plasmid