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Wash the cells with 300 l of PBS and centrifuge at 200 x g for 10 minutes at 4C

Increasing dietary fiber consumption through fruits, vegetables, legumes, and whole grains is an advantageous public health initiative (13, 14)

In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of gammaglutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 4)

Mazzotti, D

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