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Hematoxylin was used for counterstaining for 2 min, followed by differentiation with 1% hydrochloric acid solution

Caution is advised in patients with Leber optic atrophy, a genetic disorder caused by chronic cyanide intoxication (such as from smoking, alcohol or some plants), against the use of cyano-B12 due to its cyanide moiety, whilst hydroxo-B12 is a cyanide antagonist and can therefore be used in these patients

For the Th-2-31 efficacy study, 18 hamsters were used and randomized into each group

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Furthermore, some peptides may have specific side effects, which researchers must note before including in their studies