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We thus investigated the role of UBE2D3 in HCC

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For pathway reconstitution experiments, the codon-optimized BCCT gene (Aci1347) was cloned into plasmid pGEX-6P-1- cntAB using the In-Fusion cloning system (Takara) yielding construct pGEX-6P-1- cntAB-bcct

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it may also help to support normal exercise tolerance