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The mice were euthanized at the end of the experiment, and xenografted tumors were dissected, weighed, and photographed

Monitoring the disappearance of FRET in liposomes following dilution with unlabeled vesicles has been used previously to determine the dilution of the original membrane after treatment with excess of unlabeled vesicles and to demonstrate liposomal membrane fusion (39)

Kong W et al. Nat Med. 2004;10(12):1344-51. Yin J et al. Metabolism. 2008;57(5):712-7. Zhang Y et al. J Clin Endocrinol Metab. 2008;93(7):2559-2565. Yang J et al. Evid Based Complement Alternat Med. 2012;2012:363845. Perez-Rubio K et al. Metab Syndr Relat Disord. 2013;11(5):366-9. Cao C et al. Exp Ther Med. 2019;17(4):3009-3014. Lee YS et al. Diabetes. 2006;55:2256-64. Brusq JM et al. J Lipid Res. 2006;47(6):1281-8. Jiang D et al. Lipids Health Dis. 2016;15:214

Discussion In this study, we used metabolic network modelling to identify drug targets and candidate drugs for alternative melanoma treatment to be used individually or in combination with conventional targeted kinase inhibition

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