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IGFBP Proteolysis and Physiological Consequences The fact that proteolytic cleavage of IGFBP-3 is common, and may result in falsely elevated IGFBP-3 immunoactivity, is the most likely reason for observing a low IGF-I/ IGFBP-3 ratio

Published preclinical studies used intraperitoneal injection in rodents, which bypasses the subcutaneous absorption variability human protocols face