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Exosomes containing miR-124a-containing exosomes, isolated from neurons and internalized by astrocytes, result in upregulation of EAAT2 protein levels but not mRNA

BohnTBonetMLBorelPKeijerJLandrierJFMilisavIet al

When areas of the body are subject to ongoing inflammation, many issues can arise

Purification: The cut peptide chain undergoes purification treatment to remove impurities

The bacterial pellet was resuspended in LEW buffer (17.9 g Na 2 HPO 4 12H 2 O, 17.5 g NaCl, dissolve them in 800 mL of double-distilled water, adjust the pH value to 7.0 with NaOH, and then add double-distilled water to make the volume up to 1 L), and ultrasonic disruption was performed at 30 Hz for 16 min (with a cycle of 5 s of sonication and 3 s of pause)