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With respect to the methylation of these catechols, 2- and 4-MeOE2 and 4-MeOE1 levels were unchanged, while 2-MeOE1 was significantly increased in COC users

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LC-MS analyses were performed using an ultra-high performance liquid chromatography (UPLC) system (Vanquish, Thermo Fisher Scientific, United States) with an ACQUITY UPLC HSS T3 (2.1 150 mm, 1.8 m) (Waters, Milford, MA, United States) column connected to Thermo Orbitrap Exploris 120 mass spectrometry detector (Thermo Fisher Scientific, United States)