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Asmussen E, Hald J, & Larsen V (1948) The pharmacological action of acetaldehyde on the human organism

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Bio-Rad was used to transfect the brain slices with a human htt exon-1 expression construct containing a 73 CAG repeat (HttN90Q73) in the gWiz backbone (Genlantis) together with a YFP expression construct to visualize transfected neurons

These results indicated that GHK decreases AQP4 expression via miR-146a-3p through the PI3K/Akt pathway (Figure 7)

Ethanol-fixed cells were stained with propidium iodide (PI) at room temperature for 1 h in PBS containing 20 g/ml PI (Sigma-Aldrich, St