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This supports previous studies showing the most intense MT3 immunohistochemical staining arising in the spinal cord grey matter where it is prominently decreased in ALS cases 23 , in addition to a more pronounced decrease in MT3 mRNA levels in the MS spinal cord grey matter 16

In all studies, the AX iAECs alone or in co-culture with the hLMVECs were maintained and differentiated on the AX12 for three weeks prior to cigarette smoke treatment or exposure conditions, where ALI and stretch was initiated in specified experiments around 10 days prior to smoke exposure

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Salmani-Javan E, Jafari-Gharabaghlou D, Bonabi E, et al

The slides were cleaned with xylene and mounted with glass coverslips