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By counteracting mitochondrial generation of ROS GPx4 appears to protect neuronal cells from oxidative modification of AIF, which prevents AIF cleavage and release into the cytosol (Seiler et al., 2008)

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Emission intensity of the purified sensor protein diluted in 20 mM TBS buffer (pH 7.2) was investigated using Cary eclipse fluorescence spectrophotometer (Agilent, USA)

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