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Determination of total glutathione content was conducted by mixing 20 l of obtained supernatant with 30 l of 200 mM CHES (Sigma-Aldrich, C8210) buffer (pH 9.4) and 5 l of 3 mM DTT (Roth, 6908.1), followed by 1-h incubation at RT

These findings suggest that vitamin C supplementation may potentially be clinically useful to limit the deleterious effects of maternal smoking during pregnancy on offsprings lung function