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22 Diet preparation In order to explore whether dietary supplementation with LC improved liver lipid metabolism through simultaneously promoting mitochondrial -oxidation and inhibiting ER stress in large yellow croaker fed with HFD, the lipid level in diets was chosen to be 18 %, in accordance with previous studies (Reference Yan, Liao and Wang38,Reference Liao, Yan and Mai40,Reference Wang, Li and Hou47,Reference Li, Xu and Lai48)

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