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Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)

In terms of the IKK complex, an innovative small-molecule compound, SR12343, capable of inhibiting NF-B activation was recently identified, which functions by specifically disrupting the IKK-NEMO association

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This allows toxic microbial metabolites and other harmful substances to enter systemic circulation, activating the immune system and promoting the release of pro-inflammatory cytokines