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Multiple histone octamer concentrations were assayed for the same DNA concentration (x-axis) to evaluate the NCP reconstitution optimal sample (label in bold) that exhibited minimal presence of residual free DNA

Aliquots of 300 ul of the supernatant were transferred to fresh tubes for further analysis, while the remaining supernatant fluid was discarded and the protein pellet resuspended in 200 ul of 1 N NaOH and analyzed for protein quantification using the BioRad DC Assay with BSA as a standard

Epilepsy syndromes and seizures types were characterized in accordance with the classification and terminology of the International League Against Epilepsy

Polyamines, which are derived from arginine (ornithine) and decarboxylated S -adenosylmethionine [50], occur in all cells [51] and can reach high micromolar concentrations in brain tissue [52]

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