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Sequence analysis of the GCL from multiple species indicates that these enzymes group into three distinct families (Figure 3A), as follows: non-plant eukaryotes (mammals, yeast, and trypanosomes), -proteobacteria ( Escherichia coli ), and plants ( Arabidopsis ) and -proteobacteria ( Rhizobium

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Modification of Ph1-FLAG and Ph1 phage particles Ph1-FLAG phage particles (~ 10 11 ) were combined with reaction buffer (20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, pH 8.0) to a total reaction volume of 100 L