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Sies H, Klotz L-O, Sharov VS, Assmann A, Briviba K

In fact, in non cross-over design, the placebo effect will not be mixed up with the effect of the test material

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Metabolic profiling Extraction and analysis of polar metabolites were performed as previously described 90,91 , with some modifications: 100 L of plasma were mixed with 1 mL of a pre-cooled (-20 C) homogenous methanol (MetOH):methyl-tert-butyl-ether (MTBE) 1:3 (v/v) mixture

Every batch verified 99% pure by RP-HPLC and identity-confirmed by LC-MS