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However, the abrupt onset and probable insulinopenia, demonstrated by the need for insulin, suggest an autoimmune cause, which was corroborated by the autoantibody test results

Supernatant containing proteins were collected for immunoblotting, extracted proteins (2040 g) were separated by SDS-PAGE (615%) gel and then electroblotted onto Polyvinylidene Fluoride (PVDF) membranes (Amersham Hybond-P, GE-Healthcare Life Science, Pittsburgh, PA, USA)

This study was approved by the Ethics Committee for Animal Experimentation of the University of Barcelona (CEEA-UB

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Glucosinolates in human health: metabolic pathways, bioavailability, and potential in chronic disease prevention