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Yeast one-hybrid (Y1H) analysis The 1456 bp upstream sequence from +1 site of AtNRAMP3, 1500 bp upstream sequence from +1 site of both AtNRAMP4 and AtPIC1 genes were amplified by PCR and subsequently cloned into the KpnI and SacI RE sites of the pAbAi vector (Takara)

Furthermore, we offer insights into circadian rhythmregulating therapies, which may contribute to the development of novel strategies for the prevention and treatment of circadian rhythmrelated diseases