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Dlye et al

IGF-1 is synthesized by the liver, and it exerts its effect by binding with specific cell surface receptors present on various cells

This involved 100 L of serum treated with 50 L of Ellmans reagent (19.8 mg of 5,5dithiobisnitrobenzoic acid in 100 mL of 0.1% sodium citrate) and 300 L of phosphate buffer (pH 8.0)