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Human NSCLC cell lines, A549, Lu99 and EBC-1, (Riken BioResearch Center, Tsukuba, Japan) were cultured in 100-mm dishes (Becton Dickinson Labware) in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Moregate Biotech), penicillin (5 g/ml), streptomycin (5 g/ml) and neomycin (10 g/ml)

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This adaptability can make it easier for individuals to incorporate fasting into their lifestyles

Acknowledgments Footnotes References 1

471 SCFAs enhance gut barrier integrity, reducing LPS translocation and minimizing the transfer of proinflammatory substances to the liver, thereby curbing subsequent inflammatory responses