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Additionally, ApoM has been shown to increase insulin secretion by binding to bioactive sphingolipid sphingosine 1-phospate (S1P) [13] and, in turn, insulin has been shown to inhibit ApoM expression [14]

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The 650 bp fragment was further digested with Apa I to obtain a singly labeled 443 bp fragment ( Apa I 14179 - Eco RI*14621) and a 211 bp fragment ( Hin d III*13972 - Apa I 14182)