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For comparison between treatment groups, the null hypothesis was tested by either a single-factor analysis of variance (ANOVA) for multiple groups or an unpaired t-test for two groups, and the data are presented as mean SEM

Munc18-1 deficient mice were generated as described previously (de Vries et al., 2000)

Interstitial solutes are ultimately cleared into peripheral lymph nodes outside the brain parenchyma

However, significant challenges remain, including the synthesis of stable tripeptides with good tissue penetration, insufficient understanding of their mechanisms of action, the scarcity of studies in larger animal models and human trials, and the lack of direct head-to-head comparisons with larger peptides [75, 95]

U-2OS and KHOS-24OS cells were cultured in 90% RPMI-1640 containing 10% FBS