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Description

Briefly, 10 L of CCK-8 reagent (Biosharp, Hefei, China) was added to each well and then the 96-well plate was continuously incubated at 37 C for 1 h

However, whether NOX4 or other NADPH oxidases cause ferroptosis in -cells and thus induce or exacerbate diabetes needs further validation

However, the sample size of this study was small

Biochemically, it is marked by disrupted iron metabolism, accumulation of lipid hydroperoxides (LPO), and depletion of intracellular glutathione (GSH)