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The plate was cultivated at 2628 C for 7 days, and finally the excystment rate was recorded, as follows: excystment rate = (number of excysted tomonts/total number of protomonts) 100%

10.1016/S0006-8993(99)01115-4 [DOI] [PubMed] [Google Scholar] Tomita N., Morishita R., Taniyama Y., Koike H., Aoki M., Shimizu H., et al

Cell culture Porcine ovarian granulosa cell culture methods were described in detail in a previous study 59

In addition, people who have pernicious anemia (a condition caused by autoimmunity to stomach cells that produce intrinsic factor), celiac disease and other illnesses of the small intestine, those who have undergone gastrointestinal surgery, and vegans/vegetarians are also at risk for vitamin B12 deficiency

** Pour the alcohol over, then add the water