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Shah AA, Ahmed S, Ali A, Yasin NA (2020) 2-Hydroxymelatonin mitigates cadmium stress in cucumis sativus seedlings: modulation of antioxidant enzymes and polyamines

After blocking with 5% BSA for 1 h, the membranes were incubated with anti--SMA (1:1,000 dilution, Abcam, USA) anti-Nrf2 (1:1,000 dilution, Proteintech Group, Rosemont, IL, USA), anti-HO-1 (1:1,000 dilution, Proteintech Group, Rosemont, IL, USA), anti-GPX4 (1:1,000 dilution, Abmart, Shanghai, China), anti-xCT (1:1,000 dilution, Shanghai, China), anti--tubulin antibodies (1:1,000 dilution, Cell Signaling Technology, Danvers, MA, USA) or anti-Lamin-B1 antibodies (1:10,000 dilution, Proteintech Group, Rosemont, IL, USA) at 4 C overnight, followed by horseradish peroxidase-linked anti-mouse (1:1,000 dilution, Cell Signaling Technology, Danvers, MA, USA) or anti-rabbit secondary antibodies (1:2,000 dilution, Cell Signaling Technology, Danvers, MA, USA) at 37 C for 1 h

doi: 10.1038/s41575-024-01035-7

Immunity 41 (2), 296310

They act as biological messengers in the body, helping regulate vital processes like hormone release, inflammation, growth, metabolism, and cellular repair