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Studies have shown that Mn induced S-nitrosylation of PINK1, resulting in the repression of PINK1/parkin-mediated mitophagy function, ultimately leading to cell damage (Zhang et al., 2016

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ALA recycles glutathione and increases the uptake of its building blocks

Immunofluorescence staining HFF-1 cells (1.5 10 5 cells per well) or mouse embryonic fibroblasts (NIH3T3) were grown on sterile cell slides for 24 h till 80% confluence